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Solvent of vacuum degasser

Degassing, an important step in the preparation of high performance liquid chromatography mobile phases, is used to remove dissolved atmospheric gases from drilling fluid which may interfere with the normal operation of the pump and detector.

Bubbles that from when these gases come out of solution may result in loss of pump prime and other irregularities, such as inaccurate and irreproducible flow, ultimately resulting in inconsistent retention retention times and noisy or drifting chromatograpgic baselines.

A bubble in the detector’s flow cell typically manifests itself as a “spike” which may hinder peak detection and integration. Dissolved oxygen poses special problems such as baseline shifts caused by the formation of ultraviolet absorbing oxygen -solvent complexes and diminished fluorescence response.

vacuum degasser

Applying a vacuum for several minutes to a solvent reservoir is an inexpensive, effective method of degassing. It is typically performed with a side-arm filtration flask and a vacuum aspirator attached to a laboratory faucet. Alternatively, a vacuum pump may be used. Care must be taken when empoying vacuum degassing. because it is an off-line, noncontinuous process, its long-term effectiveness is limited, as air will begin to redissolve as soon as the vacuum is removed. Complete resaturation of the mobile phase will occur within several hours. It is most effective when used on premixed solvents for isocratic separations and is generally unacceptable when performing gradient separation with a low-pressure solvent proportioning device.

In addition, vigorous vacuum degassing may alter the composition of mixed solutions as the more volatil;e organic components are selectively evaporated. This may lead to variablity in the retention times of separations performed with different batches of mobile phase.

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